1.2 Learning Objectives

Introduction to Biochemistry

Draw and describe the structure of a eukaryotic gene including promoter, transcribed region & terminator.

Identify the proteins required to initiate transcription of a eukaryotic gene and where they bind.

Predict the sequence of mRNA transcribed from a particular gene sequence.

Identify the introns in pre-mRNA removed in RNA splicing, draw and label a mature mRNA, and discuss the importance of the 5’ cap and 3’ poly A tail.

Explain the principle of alternative splicing and describe how multiple proteins can be derived from a single gene.

Construct an amino acid sequence by using a genetic code chart (codon chart).

Identify the roles of the different types of RNA in the process of translation.

Discuss the importance of tRNA anticodons in the addition of the correct amino acid in elongation.

Contrast ribozymes and enzymes.

List and describe the steps involved in the initiation, elongation and termination of translation, and include the structure of ribosomes in the description (subunits, sites).

Predict the protein sequence from the coding frame of an mRNA